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Now that single-molecule detection was no longer necessary, Solexa was able to develop its signature sequencing chemistry. The process takes place on a chip called a flow cell, which contains a lawn of short DNA sequences affixed to its surface. The template DNA is broken up into small fragments, and adapter sequences, complementary to the DNA on the flow cell’s surface, are added to the ends of each fragment. DNA fragments are then passed over the flow cell, where the adapter sequences bind to spots on the DNA lawn. At this point, primers are added, and an initial round of amplification takes place: the short DNA sequences on the flow cell are extended to create sequences complementary to the bound template DNA fragments, which are then washed away. The sequences present in the fragments of template DNA are now affixed directly to the flow cell.,更多细节参见heLLoword翻译官方下载
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执行到 sleep 时,线程会进入阻塞状态,操作系统把它挂起,不分配 CPU 时间片。但这个线程仍然被占用着,线程池里它的“坑位”一直没释放。如果同时有 200 个请求进来,线程池的 200 个线程全被占满,都在等 IO,第 201 个请求就得排队,哪怕 CPU 空闲 99%。,这一点在体育直播中也有详细论述
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